Fast and Accurate SNP/Indel Calling Pipeline

• Integrated pipeline from fastq to VCF, perform alignment and variant calling;
• Output GVCF and VCF files; Supports Chemistry R10.4 and above;
• Compared to Clair3, Sentieon pipeline reduces SNP error by over 50%, reduces Indel error by 10-25%;

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Highly Accurate Structural Variants Calling Pipeline

Haplotype-resolved single base resolution SV caller

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Rapid Alignment and Variant Calling

• Sentieon Minimap2-turbo and variant caller is ~5x faster than opensource pipeline in generating VCF or GVCF files;
• Benchmark was conducted analyzing 73x HG002 dataset using 64 core Intel Xeon Gold 5218 server;
• Adding in the SV calling is negligible to the total time;